Difference between revisions of "Part:BBa K5115037"

 
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K5115037 short</partinfo>
 
<partinfo>BBa_K5115037 short</partinfo>
  
−
ribozyme+RBS+csoS4B+stem-loop
+
<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
 +
__TOC__
 +
===Introduction===
 +
This composite part consists of Tardigrade Desiccation Protein (TDP) and ''Ammopiptanthus nanus'' Antifreeze Protein (AnAFP). They were respectively tested by iGEM17_TUDelft in [https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003] and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015].
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
TDP, derived from tardigrades, is known for its ability to protect cellular structures during desiccation by forming a glass-like matrix, enabling survival in extreme dehydration. AnAFP, sourced from ''Ammopiptanthus nanus'', is an antifreeze protein that stabilizes cell membranes under cold stress by interacting with the membrane and calcium ions, preventing macromolecule aggregation. Together, these proteins enhance cellular resilience against both dehydration and freezing conditions.
 +
 +
===Characterization===
 +
 +
===Sequence and Features===
  
 
<!-- -->
 
<!-- -->
Line 17: Line 24:
 
<partinfo>BBa_K5115037 parameters</partinfo>
 
<partinfo>BBa_K5115037 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
==References==
 +
<references />

Latest revision as of 06:09, 2 October 2024


TDP+AnAFP

contributed by Fudan iGEM 2023

Introduction

This composite part consists of Tardigrade Desiccation Protein (TDP) and Ammopiptanthus nanus Antifreeze Protein (AnAFP). They were respectively tested by iGEM17_TUDelft in BBa_K2306003 and BBa_K4765015.

Usage and Biology

TDP, derived from tardigrades, is known for its ability to protect cellular structures during desiccation by forming a glass-like matrix, enabling survival in extreme dehydration. AnAFP, sourced from Ammopiptanthus nanus, is an antifreeze protein that stabilizes cell membranes under cold stress by interacting with the membrane and calcium ions, preventing macromolecule aggregation. Together, these proteins enhance cellular resilience against both dehydration and freezing conditions.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 178
    Illegal SapI.rc site found at 1047


References