Difference between revisions of "Part:BBa K5115075"
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<partinfo>BBa_K5115075 short</partinfo> | <partinfo>BBa_K5115075 short</partinfo> | ||
| − | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM | + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html> |
__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| + | The nikD is a subunit of the nik operon [https://parts.igem.org/Part:BBa_K2652006 BBa_K2652006]. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | NikD is a membrane-associated protein with ATPase activity in the NikABCDE complex. It provides the energy required for nickel transport by hydrolyzing ATP. In our design, nikD is constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115080 BBa_K5115080(ribozyme+RBS+nikD+stem-loop)], eventually making up parts [https://parts.igem.org/Part:BBa_K5115082 BBa_K5115082(ribozyme connected nik operon)], which is used for further experiments to facilitate nickel uptake in ''E. coli''. | ||
===Characterization=== | ===Characterization=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K5115075 SequenceAndFeatures</partinfo> |
Latest revision as of 05:14, 25 September 2024
nikD
Introduction
The nikD is a subunit of the nik operon BBa_K2652006.
Usage and Biology
NikD is a membrane-associated protein with ATPase activity in the NikABCDE complex. It provides the energy required for nickel transport by hydrolyzing ATP. In our design, nikD is constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115080(ribozyme+RBS+nikD+stem-loop), eventually making up parts BBa_K5115082(ribozyme connected nik operon), which is used for further experiments to facilitate nickel uptake in E. coli.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 207
- 1000COMPATIBLE WITH RFC[1000]