Difference between revisions of "Part:BBa K5115072"

 
 
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<partinfo>BBa_K5115072 short</partinfo>
 
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
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__TOC__
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===Introduction===
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The nikA is a subunit of the nik operon [https://parts.igem.org/Part:BBa_K2652006 BBa_K2652006].
  
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===Usage and Biology===
 
===Usage and Biology===
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NikA is a periplasmic nickel-binding protein, responsible for sensing and transporting nickel ions into the cell as part of the NikABCDE transporter system. In our design, NikA is constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115077 BBa_K5115077(ribozyme+RBS+nikA+stem-loop)], eventually making up parts [https://parts.igem.org/Part:BBa_K5115082 BBa_K5115082(ribozyme connected nik operon)], which is used for further experiments to facilitate nickel uptake in ''E. coli''.
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===Characterization===
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<partinfo>BBa_K5115072 parameters</partinfo>
 
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==References==
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<references />

Latest revision as of 05:12, 25 September 2024


nikA

contributed by Fudan iGEM 2024

Introduction

The nikA is a subunit of the nik operon BBa_K2652006.

Usage and Biology

NikA is a periplasmic nickel-binding protein, responsible for sensing and transporting nickel ions into the cell as part of the NikABCDE transporter system. In our design, NikA is constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115077(ribozyme+RBS+nikA+stem-loop), eventually making up parts BBa_K5115082(ribozyme connected nik operon), which is used for further experiments to facilitate nickel uptake in E. coli.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1560
  • 1000
    COMPATIBLE WITH RFC[1000]


References