Difference between revisions of "Part:BBa K5115050"

 
 
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<partinfo>BBa_K5115050 short</partinfo>
 
<partinfo>BBa_K5115050 short</partinfo>
  
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
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__TOC__
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===Introduction===
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Metallothioneins (MTA) are intracellular, low molecular, low molecular weight, cysteine-rich proteins. Ubiquitous in eukaryotes, MTA have unique structural characteristics to give potent metal-binding and redox capabilities<ref>Coyle, P., Philcox, J. C., Carey, L. C., & Rofe, A. M. (2002). Metallothionein: The multipurpose protein. Cellular and Molecular Life Sciences: CMLS, 59(4), 627–647.</ref>. Closely binding with nickel ion, MTA can reduce the harm this metal may cause to the bacteria.
  
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===Usage and Biology===
 
===Usage and Biology===
 
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In our design, MTA mainly works with  [https://parts.igem.org/Part:BBa_K5115000 BBa_K5115000(RcnR_C35L)]and  [https://parts.igem.org/Part:BBa_K1151001 BBa_K1151001(hpn)]. They can raise the absorptivity of nickel ion as well as reduce the harm nickel brings to our engineering bacteria.
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 10:29, 2 October 2024


MTA, Metallothionein

contributed by Fudan iGEM 2024

Introduction

Metallothioneins (MTA) are intracellular, low molecular, low molecular weight, cysteine-rich proteins. Ubiquitous in eukaryotes, MTA have unique structural characteristics to give potent metal-binding and redox capabilities[1]. Closely binding with nickel ion, MTA can reduce the harm this metal may cause to the bacteria.

Usage and Biology

In our design, MTA mainly works with BBa_K5115000(RcnR_C35L)and BBa_K1151001(hpn). They can raise the absorptivity of nickel ion as well as reduce the harm nickel brings to our engineering bacteria.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 123
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. Coyle, P., Philcox, J. C., Carey, L. C., & Rofe, A. M. (2002). Metallothionein: The multipurpose protein. Cellular and Molecular Life Sciences: CMLS, 59(4), 627–647.