Difference between revisions of "Part:BBa K243029:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Glycine and serine are good suitable for repetitive sequences.The difference to our [https://parts.igem.org/Part:BBa_K243030 GSATLinker] is that the sequence produce some glutamic acid and alanin amino acids.The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br>
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Glycine and serine are very suitable for repetitive sequences. The difference to our [https://parts.igem.org/Part:BBa_K243030 GSATLinker] is that the sequence produced contains some glutamic acid and alanin amino acids. The properties zwitterionic and hydrophile prevent the aggregation of the linker.<br>The sequence has no cleavage site for proteases. <br>
 
[[Image:Freiburg09 Gsatpart.png|750x550px]]<br>
 
[[Image:Freiburg09 Gsatpart.png|750x550px]]<br>
 
[https://static.igem.org/mediawiki/parts/a/a4/Freiburg09_GSAT.txt Commented GenBank file]
 
[https://static.igem.org/mediawiki/parts/a/a4/Freiburg09_GSAT.txt Commented GenBank file]

Latest revision as of 21:59, 21 October 2009

GSAT Linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Glycine and serine are very suitable for repetitive sequences. The difference to our GSATLinker is that the sequence produced contains some glutamic acid and alanin amino acids. The properties zwitterionic and hydrophile prevent the aggregation of the linker.
The sequence has no cleavage site for proteases.
Freiburg09 Gsatpart.png
Commented GenBank file

Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.

Source

Planed and designed by Team Freiburg Bioware and synthesized by Mr.Gene.

References