Difference between revisions of "Part:BBa K5115009"

Latest revision as of 13:13, 2 October 2024

hypB

It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system BBa_K5115018(ribozyme+RBS+hypB+stem-loop). And then it will eventually make up parts BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module), which are used for further experiments.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 336
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 25
Illegal NgoMIV site found at 871
Illegal AgeI site found at 823
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5115009 short</partinfo>
-hypB
+<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2024"></html>
+__TOC__
+===Introduction===
+The hypB is a subunit of the hox and hyp operon [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+It's used to build up the hydrogenase which reduces the nickel ion. In our experiment, it was constructed into our ribozyme-assisted polycistronic co-expression system [https://parts.igem.org/Part:BBa_K5115018 BBa_K5115018(ribozyme+RBS+hypB+stem-loop)]. And then it will eventually make up parts [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)], which are used for further experiments.
+===Sequence and Features===
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
@@ Line 17: / Line 21: @@
 <partinfo>BBa_K5115009 parameters</partinfo>
 <!-- -->
+==References==
+<references />