Difference between revisions of "Part:BBa K257010"
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Insertion of the protein fusion clyA-RFP in the outer membrane of the cell. During vesiculation process, we can observe vesicles by fluorescence. | Insertion of the protein fusion clyA-RFP in the outer membrane of the cell. During vesiculation process, we can observe vesicles by fluorescence. | ||
| + | |||
| + | === Safety Note === | ||
| + | ClyA (hlyE) is a toxin. For more information see here: <partinfo>BBa_K257002</partinfo> | ||
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Latest revision as of 07:23, 22 October 2009
clyA + RFP
Insertion of the protein fusion clyA-RFP in the outer membrane of the cell. During vesiculation process, we can observe vesicles by fluorescence.
Safety Note
ClyA (hlyE) is a toxin. For more information see here: BBa_K257002
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 84
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1494
Illegal AgeI site found at 1606 - 1000COMPATIBLE WITH RFC[1000]