Difference between revisions of "Part:BBa K201006:Design"

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===Design Notes===
 
===Design Notes===
  
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This part was designed with the same structure of the Berkley constitutive promoter family. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated.  
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This part was designed with the same structure of the [https://parts.igem.org/Part:BBa_J23100 Berkley constitutive promoters]. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated.  
 
This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp).
 
This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp).
  

Latest revision as of 14:04, 21 October 2009

Lac Osym natural operator with RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed with the same structure of the Berkley constitutive promoters. Indeed, the operator sequence was digested with SpeI and PstI and the same was done for the RFP. The two parts were then ligated. This was done to allow an easier cloning of the operator sequence that is short in length (about 20 bp).

Source

All subparts come from the Registry of Standard Parts


References