Difference between revisions of "Part:BBa K5492020"
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Forward primer sequence of the adapter sequence utilised by GenScript. | Forward primer sequence of the adapter sequence utilised by GenScript. | ||
| + | Utilised in PCR reactions for part [https://parts.igem.org/Part:BBa_K5492402 BBa_K5492402] and [https://parts.igem.org/Part:BBa_K5492403 BBa_K5492403]. | ||
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| − | + | ===Experiments=== | |
| − | + | ==PCR – HNMT== | |
| + | We have found the following PCR protocol the most optimal for the part: | ||
| + | https://static.igem.wiki/teams/5492/registry/hnmt-pcr-plan.png | ||
| + | According to the previous PCR reaction we decided to use S1 and N1 (from our previous | ||
| + | reactions.) | ||
| + | We added the following substrates in order: (each optimized and wild type) | ||
| + | |||
| + | https://static.igem.wiki/teams/5492/registry/hnmt-pcr-substr1.png | ||
| + | |||
| + | We added 6X DNA Loading dye to each PCR tubes. | ||
| + | We loaded the wells of the gels with 12μL of DNA solution in the following order: | ||
| + | |||
| + | https://static.igem.wiki/teams/5492/registry/hnmt-pcr.png | ||
| + | 1. S1O (optimized) | ||
| + | 2. S1W (wild type) | ||
| + | 3. N1O (optimized) | ||
| + | 4. N1W (wild type) | ||
| + | 5. Ladder | ||
| + | <partinfo>BBa_K5492021 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 11:38, 2 October 2024
Genscript adapter forward primer
Forward primer sequence of the adapter sequence utilised by GenScript. Utilised in PCR reactions for part BBa_K5492402 and BBa_K5492403.
Experiments
PCR – HNMT
We have found the following PCR protocol the most optimal for the part:
According to the previous PCR reaction we decided to use S1 and N1 (from our previous reactions.) We added the following substrates in order: (each optimized and wild type)
We added 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DNA solution in the following order:
1. S1O (optimized)
2. S1W (wild type)
3. N1O (optimized)
4. N1W (wild type)
5. Ladder
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]