Difference between revisions of "Part:BBa K5492011"

Latest revision as of 15:03, 1 October 2024

Twist adapter reverse primer

Reverse primer sequence of the adapter sequence utilised by Twist Bioscience. Utilised in PCR reactions for part BBa_K5492202 and BBa_K5492203.

We have found the following PCR protocol the most optimal for the part:

We added the following substrates in order:

We added 6X DNA Loading dye to each PCR tubes. We loaded the wells of the gels with 12μL of DAO solution in the following order:

1.D31O 2.D32O 3.D33O 4.D34O 5.Ladder

Assembly Compatibility:

@@ Line 4: / Line 4: @@
 Reverse primer sequence of the adapter sequence utilised by Twist Bioscience.
+Utilised in PCR reactions for part [https://parts.igem.org/Part:BBa_K5492202 BBa_K5492202] and [https://parts.igem.org/Part:BBa_K5492203 BBa_K5492203].
 <!-- Add more about the biology of this part here
@@ Line 9: / Line 10: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Experiments===
+==PCR - optimised DAO==
+We have found the following PCR protocol the most optimal for the part:
+https://static.igem.wiki/teams/5492/registry/dao-pcr-plan.png
+We added the following substrates in order:
+https://static.igem.wiki/teams/5492/registry/dao-pcr-substr.png
+We added 6X DNA Loading dye to each PCR tubes.
+We loaded the wells of the gels with 12μL of DAO solution in the following order:
+https://static.igem.wiki/teams/5492/registry/opt-dao-pcr.png
+.D31O 2.D32O 3.D33O 4.D34O 5.Ladder
 <partinfo>BBa_K5492011 SequenceAndFeatures</partinfo>