Difference between revisions of "Part:BBa K5492722:Design"
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===Design Notes===
 
===Design Notes===
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complementary part to BBa_K5492732 (H5_SULIVAN_REVCOMP)
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[https://parts.igem.org/Part:BBa_K5492712 s1 ssDNA aptamer] sequence designed to specifically bind to histamine.
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fluorophor /5cy5/ is attached to the start of the sequence
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The sequence contains a fluorophore molecule at its 5' end.
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[https://parts.igem.org/Part:BBa_K5492732 s1_reverse_compliment_strand] is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by fluorophore until the aptamer binds histamine.
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===Source===
 
===Source===
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===References===
 
===References===
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http://www.unafold.org/mfold/applications/dna-folding-form.php - Folding
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Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.
 
Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.

Latest revision as of 21:48, 1 October 2024


S1_aptamer_with_fluorophore


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

s1 ssDNA aptamer sequence designed to specifically bind to histamine. The sequence contains a fluorophore molecule at its 5' end. s1_reverse_compliment_strand is complementary to this sequnce, which contains a quencher molecule which absorbs the light emitted by fluorophore until the aptamer binds histamine.

Source

https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b00075 Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.

References

http://www.unafold.org/mfold/applications/dna-folding-form.php - Folding

Reference: Zhang, H.; Lai, Y.; Wang, L.; Chen, Z.; Wang, Z. High-Throughput Screening of DNA Aptamers for Bisphenol A Detection Based on Circular Dichroism and Fluorescence. Anal. Chem. 2019, 91 (10), 6581–6588. DOI: 10.1021/acs.analchem.9b00075.