Difference between revisions of "Part:BBa K243005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Glycine and serine are | + | Glycine and serine are very suitable for repetitive sequences. Their properties zwitterionic and hydrophile prevent aggregation of the linker.<br> |
The sequence has no cleavage site for proteases. <br> | The sequence has no cleavage site for proteases. <br> | ||
[[Image:Freiburg09 Middlelipart.png|750x550px]] <br> | [[Image:Freiburg09 Middlelipart.png|750x550px]] <br> | ||
| − | Designed for the fusion of proteins or peptides via in frame cloning, according to | + | Designed for the fusion of proteins or peptides via in frame cloning, according to [https://parts.igem.org/Assembly_standard_25 RFC 25].<br> |
| − | + | ||
[https://static.igem.org/mediawiki/parts/b/bd/MiddleLinker.txt Commented GenBank file] | [https://static.igem.org/mediawiki/parts/b/bd/MiddleLinker.txt Commented GenBank file] | ||
===Source=== | ===Source=== | ||
| − | + | Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR. | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 20:48, 21 October 2009
Middle Linker ( Gly-Gly-Ser-Gly)x2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Glycine and serine are very suitable for repetitive sequences. Their properties zwitterionic and hydrophile prevent aggregation of the linker.
The sequence has no cleavage site for proteases.
Designed for the fusion of proteins or peptides via in frame cloning, according to RFC 25.
Commented GenBank file
Source
Oligonucleotides designed by Team Freiburg Bioware09 and synthesized by sigma. Hybridized by PCR.