Difference between revisions of "Part:BBa K5143020"
 
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ScCBD_cex'V1 : a Cellulose Binding Domain optimised for Saccharomyces cerevisiae, that enables the fixation of proteins to Bacterial Cellulose
 
 
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     <h1>Description</h1>
 
     <h1>Description</h1>
 
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         ScCBD_cex'V1 (Cellulose Binding Domain) is a peptide that has a huge affinity for bacterial cellulose. When fused with another protein, ScCBD_cex'V1 enables the fixation of the protein on the bacterial cellulose.
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         AGA2 is a pre-peptide that is secreted by the <i>Saccharomyces cerevisiae</i> yeast. When fused with another protein, AGA2 enables the secretion of the whole protein in the extracellular medium.
 
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             <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-1.png" width="200" alt="CBD_CexV1">
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             <img src="https://static.igem.wiki/teams/5143/bba-k5143021-aga2-1.png" width="200" alt="AGA2">
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             <figcaption>Figure 1: ScCBD_Cex'V1 three-dimensional structure</figcaption>
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             <figcaption><i><u>Figure 1:</u> AGA2 coding sequence</i></figcaption>
 
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             <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-2.png" width="400" alt="Functional cellulose">
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             <img src="https://static.igem.wiki/teams/5143/bba-k5143021-mruby-microscpoe.png" width="400" alt="The use of AGA2">
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             <figcaption>Figure 2: Functional cellulose</figcaption>
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             <figcaption><i><u>Figure 2:</u> The fluorescence of mRuby is assessed in either the supernatant or within the cells to determine whether the protein is produced and secreted by AGA2</i></figcaption>
 
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143007-cbd-cexv1-3.png" width="100" alt="ScCBD_Cex coding sequence">
 
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            <figcaption>Figure 3: ScCBD_Cex coding sequence</figcaption>
 
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     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
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       ScCBD-Cex'V1 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a yellow chromoprotein (fwYellow) in order to color the bacterial cellulose in yellow: <a href="https://parts.igem.org/Part:BBa_K5143023" target="_blank">BBa_K5143023</a>
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       AGA2 was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>. This protein is used in fusion with the mRuby chromoprotein in order to color the yeast culture in red and so to test the activation of the pADH1 promoter: <a href="https://parts.igem.org/Part:BBa_K5143021" target="_blank">BBa_K5143021</a>
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    <h1>References</h1>
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        Cappellaro, C. et al. Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction. The EMBO Journal 10, 4081–4088 (1991)
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<h1>Sequence and Features</h1>
 
<partinfo>BBa_K5143007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5143007 SequenceAndFeatures</partinfo>
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<br>
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<h1>References</h1>
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        Cappellaro, C. et al. <i>Saccharomyces cerevisiae</i> a- and alpha-agglutinin: characterization of their molecular interaction. The EMBO Journal 10, 4081–4088 (1991) https://doi.org/10.1002/j.1460-2075.1991.tb04984.x
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Latest revision as of 14:03, 1 October 2024


AGA2, a pre-peptide that is secreted by the Saccharomyces cerevisiae yeast

Protein Description

Description

AGA2 is a pre-peptide that is secreted by the Saccharomyces cerevisiae yeast. When fused with another protein, AGA2 enables the secretion of the whole protein in the extracellular medium.

AGA2
Figure 1: AGA2 coding sequence
The use of AGA2
Figure 2: The fluorescence of mRuby is assessed in either the supernatant or within the cells to determine whether the protein is produced and secreted by AGA2

Construction

AGA2 was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with the mRuby chromoprotein in order to color the yeast culture in red and so to test the activation of the pADH1 promoter: BBa_K5143021


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Cappellaro, C. et al. Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction. The EMBO Journal 10, 4081–4088 (1991) https://doi.org/10.1002/j.1460-2075.1991.tb04984.x