Difference between revisions of "Part:BBa K191006"
 
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<partinfo>BBa_K191006 short</partinfo>
 
<partinfo>BBa_K191006 short</partinfo>
  
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Part BBa_K191006 contains coding sequence of the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.
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Part BBa_K191006 contains coding sequence of the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain (Lov means : light, oxygen, voltage) and a regulatory domain (part of the Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site. The activation of the LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm
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For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] on team EPF-Lausanne wiki.
  
 
For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP Introduction LovTAP] on team EPF-Lausanne wiki
 
For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP Introduction LovTAP] on team EPF-Lausanne wiki
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=== Improvements ===
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[[Part:BBa_K838000]]. An implementation of the LovTAP protein for mammalian cells.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:20, 27 September 2012

LOVTAP

Part BBa_K191006 contains coding sequence of the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain (Lov means : light, oxygen, voltage) and a regulatory domain (part of the Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site. The activation of the LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm

For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] on team EPF-Lausanne wiki.

For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP Introduction LovTAP] on team EPF-Lausanne wiki

Improvements

Part:BBa_K838000. An implementation of the LovTAP protein for mammalian cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 141
    Illegal PstI site found at 398
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 535