Difference between revisions of "Part:BBa K5492202:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Restriction sites adapted to | + | Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | The work of iGEM20_SEHS-China | + | The work of iGEM20_SEHS-China [https://parts.igem.org/Part:BBa_K3684002 (BBa_K3684002)] provided the dna sequence of rDAO. |
===References=== | ===References=== | ||
| + | https://www.twistbioscience.com/faq/gene-synthesis/what-are-adapter-sequences-used-adapter-gene-fragments | ||
Latest revision as of 17:01, 26 September 2024
Wild type rDAO - pETDUET-1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 40
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 40
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 40
Illegal BglII site found at 1050
Illegal BglII site found at 2303
Illegal BamHI site found at 1978
Illegal BamHI site found at 2232
Illegal XhoI site found at 848 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 40
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 40
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.
Source
The work of iGEM20_SEHS-China (BBa_K3684002) provided the dna sequence of rDAO.