Difference between revisions of "Part:BBa J07006"
 
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malE - a monomer. This monomeric protein is used as a negative control when testing for dimerization.
 
malE - a monomer. This monomeric protein is used as a negative control when testing for dimerization.
  
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===Usage and Biology===
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===Information contributed by City of London UK (2021)===
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-- Please enter your experience with this part here --  
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Part information is collated here to help future users of the BioBrick registry.
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Metadata:
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*'''Group:''' City of London UK 2021
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*'''Author:''' Lucas Ng
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*'''Summary:''' Added information collated from existing scientific studies
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----
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It is the solute-binding protein member of the ABC transporter complex MalEFGK involved in cell chemotaxis via binding to and actively importing maltodextrins
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<ref>Davidson, A. L., and H. Nikaido. 1991. “Purification and Characterization of the Membrane-Associated Components of the Maltose Transport System from Escherichia Coli.” The Journal of Biological Chemistry 266 (14): 8946–51. https://pubmed.ncbi.nlm.nih.gov/2026607.
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</ref>.
  
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<span class='h3bb'>Sequence and Features</span>
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{| class="wikitable" style="margin-left: auto; margin-right: auto; border: none; width: 40vw; text-align: center;"
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<partinfo>BBa_J07006 SequenceAndFeatures</partinfo>
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|+ Kinetics
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<ref>Szmelcman, S., M. Schwartz, T. J. Silhavy, and W. Boos. 1976. “Maltose Transport in Escherichia Coli K12. A Comparison of Transport Kinetics in Wild-Type and Lambda-Resistant Mutants as Measured by Fluorescence Quenching.” European Journal of Biochemistry 65 (1): 13–19. https://doi.org/10.1111/j.1432-1033.1976.tb10383.x.
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</ref>
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|-
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! Maltodextrin !! K<sub>M</sub> (µM)
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|-
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| Maltose || 0.9
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| Maltriose || 2
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|}
  
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===Functional Parameters===
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Its native operator region is induced by maltose and the transcriptional regulator MalT
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<partinfo>BBa_J07006 parameters</partinfo>
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<ref>Kellermann, O., and S. Szmelcman. 1974. “Active Transport of Maltose in Escherichia Coli K12. Involvement of a ‘Periplasmic’ Maltose Binding Protein.” European Journal of Biochemistry 47 (1): 139–49. https://doi.org/10.1111/j.1432-1033.1974.tb03677.x.
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</ref>,
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while being PNPase-dependently and irreversibly repressed by cold shock
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<ref>Polissi, Alessandra, Walter De Laurentis, Sandro Zangrossi, Federica Briani, Vera Longhi, Graziano Pesole, and Gianni Dehò. 2003. “Changes in Escherichia Coli Transcriptome during Acclimatization at Low Temperature.” Research in Microbiology 154 (8): 573–80. https://doi.org/10.1016/s0923-2508(03)00167-0.
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</ref>.
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===References===

Latest revision as of 16:58, 27 July 2021


malE

malE - a monomer. This monomeric protein is used as a negative control when testing for dimerization.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

It is the solute-binding protein member of the ABC transporter complex MalEFGK involved in cell chemotaxis via binding to and actively importing maltodextrins [1].

Kinetics [2]
Maltodextrin KM (µM)
Maltose 0.9
Maltriose 2

Its native operator region is induced by maltose and the transcriptional regulator MalT [3], while being PNPase-dependently and irreversibly repressed by cold shock [4].

References

  1. ↑ Davidson, A. L., and H. Nikaido. 1991. “Purification and Characterization of the Membrane-Associated Components of the Maltose Transport System from Escherichia Coli.” The Journal of Biological Chemistry 266 (14): 8946–51. https://pubmed.ncbi.nlm.nih.gov/2026607.
  2. ↑ Szmelcman, S., M. Schwartz, T. J. Silhavy, and W. Boos. 1976. “Maltose Transport in Escherichia Coli K12. A Comparison of Transport Kinetics in Wild-Type and Lambda-Resistant Mutants as Measured by Fluorescence Quenching.” European Journal of Biochemistry 65 (1): 13–19. https://doi.org/10.1111/j.1432-1033.1976.tb10383.x.
  3. ↑ Kellermann, O., and S. Szmelcman. 1974. “Active Transport of Maltose in Escherichia Coli K12. Involvement of a ‘Periplasmic’ Maltose Binding Protein.” European Journal of Biochemistry 47 (1): 139–49. https://doi.org/10.1111/j.1432-1033.1974.tb03677.x.
  4. ↑ Polissi, Alessandra, Walter De Laurentis, Sandro Zangrossi, Federica Briani, Vera Longhi, Graziano Pesole, and Gianni Dehò. 2003. “Changes in Escherichia Coli Transcriptome during Acclimatization at Low Temperature.” Research in Microbiology 154 (8): 573–80. https://doi.org/10.1016/s0923-2508(03)00167-0.