Difference between revisions of "Part:BBa K5407001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | The HIPPO pathway is a key regulatory pathway in tumor development, with TEAD4 (TEA Domain Family Member 4) being a core transcription factor that promotes the growth and spread of CRC cancer cells [1]. si-TEAD4-1 sequence are designed to target to the coding sequence(CDS) of TEAD4. When siRNA reach the cell cytoplasm, it could binds with the RNA-induced silencing complex (RISC) [2]. Binding with the RISC leads to degradation of the sense strand, whereas the antisense strand continues to be incorporated in the RISC [3]. The RISC then makes multiple mRNA cleavages to downregulate TEAD4 gene expression. | ||
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| + | ===Design=== | ||
| + | We designed the siRNA against TEAD4 by a special database (https://www.invivogen.com/sirnawizard/). After we input the TEAD4 gene name and sequence, the database would output all the possible siRNA sequence (Figure 1). We chose two pairs of siRNAs with the least off-target possibility. | ||
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| + | https://static.igem.wiki/teams/5407/in.png | ||
| + | Figure 1 Design of siRNA Sequences Targeting TEAD4 | ||
| + | ===Reference=== | ||
| + | Reference | ||
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| + | [1] Evelien, Dekker,Pieter J, Tanis,Jasper L A, Vleugels et al. Colorectal cancer.[J] .Lancet, 2019, 394: 0. | ||
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| + | [2] Shigeyoshi, Kijima,Takahiro, Sasaki,Koichi, Nagata et al. Preoperative evaluation of colorectal cancer using CT colonography, MRI, and PET/CT.[J] .World J Gastroenterol, 2014, 20: 0. | ||
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| + | [3] Robert H, Hollis,Daniel I, Chu,Healthcare Disparities and Colorectal Cancer.[J] .Surg Oncol Clin N Am, 2022, 31: 0. | ||
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Latest revision as of 11:51, 1 October 2024
si-TEAD4-1 sequence
Usage and Biology
The HIPPO pathway is a key regulatory pathway in tumor development, with TEAD4 (TEA Domain Family Member 4) being a core transcription factor that promotes the growth and spread of CRC cancer cells [1]. si-TEAD4-1 sequence are designed to target to the coding sequence(CDS) of TEAD4. When siRNA reach the cell cytoplasm, it could binds with the RNA-induced silencing complex (RISC) [2]. Binding with the RISC leads to degradation of the sense strand, whereas the antisense strand continues to be incorporated in the RISC [3]. The RISC then makes multiple mRNA cleavages to downregulate TEAD4 gene expression.
Design
We designed the siRNA against TEAD4 by a special database (https://www.invivogen.com/sirnawizard/). After we input the TEAD4 gene name and sequence, the database would output all the possible siRNA sequence (Figure 1). We chose two pairs of siRNAs with the least off-target possibility.
Figure 1 Design of siRNA Sequences Targeting TEAD4
Reference
Reference
[1] Evelien, Dekker,Pieter J, Tanis,Jasper L A, Vleugels et al. Colorectal cancer.[J] .Lancet, 2019, 394: 0.
[2] Shigeyoshi, Kijima,Takahiro, Sasaki,Koichi, Nagata et al. Preoperative evaluation of colorectal cancer using CT colonography, MRI, and PET/CT.[J] .World J Gastroenterol, 2014, 20: 0.
[3] Robert H, Hollis,Daniel I, Chu,Healthcare Disparities and Colorectal Cancer.[J] .Surg Oncol Clin N Am, 2022, 31: 0.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]