Difference between revisions of "Part:BBa K5036002"

Latest revision as of 13:05, 2 October 2024

NLS

Part Description

It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to dCas9(C) in the first chain of dCas9(C)-TF-synVEGFR1 receptor.

Usage

This part is attached to dCas9(C) so after activation of the receptor and dimerization of the two domains of dCas9, NLS will direct the (dCas9-VP64-GAL4- UAS Trans CMV enhancer) complex into the nucleus.

this figure illustrates the structure of nuclear localization signal (NLS) which is attached to dCas9(c) in dCas9(C)-TF-synVEGFR1 receptor's first chain. .

Experimental Characterization

we digested dCas9(C)_NLS-Syn-VEGFR-1 grafetd with NLS and performed gel electrophoresis to detect the digested fragment.

This figure illustrates the digested dCas9(C)_NLS-Syn-VEGFR-1 prior to insert ligation .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5036002 short</partinfo>
 ==Part Description==
-It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to  dCas9(C) in the first chain of dCas9 synRTK receptor.
+It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to dCas9(C) in the first chain of dCas9(C)-TF-synVEGFR1 receptor.
 ==Usage==
-This part is attached to dCas9(C) so Upon dimerization of the two domains of dCas9 , NLS will direct the dCas9-VP64 complex to the nucleus
+This part is attached to dCas9(C) so after activation of the receptor and dimerization of the two domains of dCas9, NLS will direct the (dCas9-VP64-GAL4- UAS Trans CMV enhancer) complex into the nucleus.
 <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              	max-width:850px;
 							width:75%;
@@ Line 19: / Line 19: @@
 ">
 <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
-lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of nuclear localization signal (NLS)
+lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of nuclear localization signal (NLS) which is attached to dCas9(c) in dCas9(C)-TF-synVEGFR1 receptor's first chain.
+.   </span></p></div></html>
+==Experimental Characterization==
+we digested dCas9(C)_NLS-Syn-VEGFR-1 grafetd with NLS and performed gel electrophoresis to detect the digested fragment.
+<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              	max-width:850px;
+							width:75%;
+							height:auto;
+								position: relative;
+							top: 50%;
+							left: 45%;
+							transform: translate( -50%);
+							padding-bottom:25px;
+							padding-top:25px;
+							"src="https://static.igem.wiki/teams/5036/lab/sos.png
+">
+<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
+lang=EN style='font-size:11.0pt;line-height:115%'>This figure illustrates the digested dCas9(C)_NLS-Syn-VEGFR-1 prior to insert ligation
 .   </span></p></div></html>