Difference between revisions of "Part:BBa K5398612"

 
 
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The Ribonucleic Antiterminator (RAT) sequences are specialized nucleotide motifs that play a crucial role in the regulation of gene expression. It is involved in the process of transcription termination. By interacting with antitermination proteins, RAT sequences form a stem-loop structure which could prevent the formation of an RNA terminator stem-loop structure, allowing the RNA polymerase to express the reporter gene sequentially.
 
The Ribonucleic Antiterminator (RAT) sequences are specialized nucleotide motifs that play a crucial role in the regulation of gene expression. It is involved in the process of transcription termination. By interacting with antitermination proteins, RAT sequences form a stem-loop structure which could prevent the formation of an RNA terminator stem-loop structure, allowing the RNA polymerase to express the reporter gene sequentially.
  
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            border: 1px solid #ccc; /* 边框 */
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        <img src="https://static.igem.wiki/teams/5398/improvement/20240930-130531.jpg" width="500" height="auto" alt="Improved EL222 system">
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        <p><b> Fig. 1 | Improved EL222 system with <i>LicV</i> and <i>RAT</i>. </b></p>
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===Usage and Biology===
 
===Usage and Biology===
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<p>The EL222-based blue light-inducible system allows precise control of gene expression by dimerizing and binding DNA upon blue light exposure. To reduce system leakage, a LicV fusion protein and RAT sequence were introduced, enhancing control by preventing premature transcription termination. This system is ideal for applications requiring tight regulation of gene expression in response to light, such as biosensors and metabolic engineering.</p>
  
 
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Latest revision as of 13:22, 2 October 2024


Ribonucleic antiterminator (RAT)

The Ribonucleic Antiterminator (RAT) sequences are specialized nucleotide motifs that play a crucial role in the regulation of gene expression. It is involved in the process of transcription termination. By interacting with antitermination proteins, RAT sequences form a stem-loop structure which could prevent the formation of an RNA terminator stem-loop structure, allowing the RNA polymerase to express the reporter gene sequentially.

Improved EL222 system

Fig. 1 | Improved EL222 system with LicV and RAT.

Usage and Biology

The EL222-based blue light-inducible system allows precise control of gene expression by dimerizing and binding DNA upon blue light exposure. To reduce system leakage, a LicV fusion protein and RAT sequence were introduced, enhancing control by preventing premature transcription termination. This system is ideal for applications requiring tight regulation of gene expression in response to light, such as biosensors and metabolic engineering.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]