Difference between revisions of "Part:BBa K5035002:Design"

 
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
Codon optimized for E.coli
 
Codon optimized for E.coli
−
 
+
N terminal His tag added
  
  

Latest revision as of 00:03, 12 September 2024


T7RdhA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 403
    Illegal EcoRI site found at 817
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for E.coli N terminal His tag added


Source

Reference paper

References