Difference between revisions of "Part:BBa K191003"

Latest revision as of 21:20, 24 October 2009

Promoter Lac I - RBS - LovTAP - Term

Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain (Lov means : light, oxygen, voltage) and a regulatory domain (part of the Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore resulting in an increase or decrease of the regulatory domain's affinity for its DNA binding site. The activation of the LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm

For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] on team EPF-Lausanne wiki.

Usage and Biology

In order to be able to test the efficiency of the fused protein LovTAP, its expression is induced by IPTG (thanks to the LacI promoter). Two other parts were also constructed as the read out systems of LovTAP : BBa_K191004 and BBa_K191005

All three parts together give the following system, where LovTAP activities can be measured through RFP fluorescence intensity (both with with static and kinetic measurements)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 370
Illegal PstI site found at 627
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 370
Illegal PstI site found at 627
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 370
Illegal PstI site found at 627
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 370
Illegal PstI site found at 627
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 764

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K191003 short</partinfo>
-Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain ( Lov means light, oxygen, voltage)and a regulatory domain (like Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore results in an increase or decrease of the regulatory domain's affinity for its DNA binding site.
+Part BBa_K191003 codes for the hybrid protein LovTAP. This hybrid protein has a light-sensitive domain (Lov means : light, oxygen, voltage) and a regulatory domain (part of the Trp repressor). The fused protein allows transmission of the conformational change induced by light (on the light-sensitive domain) to the DNA-binding domain, therfore resulting in an increase or decrease of the regulatory domain's affinity for its DNA binding site. The activation of the LovTAP protein (binding to DNA) is achieved by illumination @ 470 nm
 For more details, please consult the section of [http://2009.igem.org/Team:EPF-Lausanne/LOVTAP LovTAP system ] on team EPF-Lausanne wiki.
 ===Usage and Biology===
-In order to be able to test the efficiency of the fused protein LovTAP,  its expression is induced by IPTG ( thanks to the LacI promoter).
+In order to be able to test the efficiency of the fused protein LovTAP, its expression is induced by IPTG (thanks to the LacI promoter).
 Two other parts were also constructed as the read out systems of LovTAP : <partinfo>BBa_K191004</partinfo> and <partinfo>BBa_K191005</partinfo>
-All three parts together gives the following system, where LovTAP activities can be measured through RFP fluorescence intensity
+All three parts together give the following system, where LovTAP activities can be measured through RFP fluorescence intensity (both with with static and kinetic measurements)
 [[Image: systems.jpg|800 px|center]]