Difference between revisions of "Part:BBa K5036002"

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<partinfo>BBa_K5036002 short</partinfo>
 
<partinfo>BBa_K5036002 short</partinfo>
 
==Part Description==
 
==Part Description==
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It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to  dCas9(C) to keep it in the nucleus to avoid dCas9 self assembly.
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It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to  dCas9(C) in the first chain of dCas9 synRTK receptor.
 +
 
 
==Usage==
 
==Usage==
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This part is attached to dCas9(C) so Upon dimerization of the two domains of dCas9 , NLS will direct the dCas9-VP64 complex to the nucleus
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This part is attached to dCas9(C) so after activation of the receptor and dimerization of the two domains of dCas9 , NLS will direct the (dCas9-VP64-GAL4- UAS Trans CMV enhancer) complex into the nucleus.
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:75%;
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height:auto;
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position: relative;
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top: 50%;
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left: 35%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/5036/parts/nls-attia.png
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">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of nuclear localization signal (NLS) which is attached to dCas9(c) in dCas9 synRTK receptor's first chain.
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.  </span></p></div></html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:42, 13 September 2024


NLS

Part Description

It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to dCas9(C) in the first chain of dCas9 synRTK receptor.

Usage

This part is attached to dCas9(C) so after activation of the receptor and dimerization of the two domains of dCas9 , NLS will direct the (dCas9-VP64-GAL4- UAS Trans CMV enhancer) complex into the nucleus.

this figure illustrates the structure of nuclear localization signal (NLS) which is attached to dCas9(c) in dCas9 synRTK receptor's first chain. .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]