Difference between revisions of "Part:BBa K5101000:Design"
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===Design Notes===
 
===Design Notes===
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In order to ensure that the novel antimicrobial peptide of human origin can be successfully expressed in E. coli EcN, we performed codon optimization. Meanwhile, we selected different promoters for gene expression to ensure the feasibility of plasmid design.
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In order to ensure that the novel antimicrobial peptide of human origin can be successfully expressed in EcN, we performed codon optimization. Meanwhile, we selected different promoters for gene expression to ensure the feasibility of plasmid design.
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+
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===Source===
 
===Source===
  
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The novel antimicrobial peptide is discovered from the <i>Lactobacilli<i> spp. in the human vaginal flora. Since the sequence screening of this protein was done by our host lab of Nanjing Tech University upfront and the related papers and data have not been published yet.
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The antimicrobial peptide is discovered from the <i>Lactobacillus</i> spp. in the human vaginal flora. The sequence screening of this protein was done by our host lab of Nanjing Tech University upfront and the related papers and data have not been published yet.
 
For reference, the sequence has been identified and is available in the NCBI database under the Reference Sequence: WP_049159833.1.
 
For reference, the sequence has been identified and is available in the NCBI database under the Reference Sequence: WP_049159833.1.
  
 
===References===
 
===References===
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[1] N. C. f. B. Information. NCBI Reference Sequence: WP_049159833.1.

Latest revision as of 12:45, 11 September 2024


Antimicrobial peptide discovered from the Lactobacillus spp. in the human vaginal flora


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to ensure that the novel antimicrobial peptide of human origin can be successfully expressed in EcN, we performed codon optimization. Meanwhile, we selected different promoters for gene expression to ensure the feasibility of plasmid design.

Source

The antimicrobial peptide is discovered from the Lactobacillus spp. in the human vaginal flora. The sequence screening of this protein was done by our host lab of Nanjing Tech University upfront and the related papers and data have not been published yet. For reference, the sequence has been identified and is available in the NCBI database under the Reference Sequence: WP_049159833.1.

References

[1] N. C. f. B. Information. NCBI Reference Sequence: WP_049159833.1.