Difference between revisions of "Part:BBa K5036002"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K5036002 short</partinfo> | <partinfo>BBa_K5036002 short</partinfo> | ||
+ | ==Part Description== | ||
+ | It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to dCas9(C) in the first chain of dCas9(C)-TF-synVEGFR1 receptor. | ||
− | + | ==Usage== | |
+ | This part is attached to dCas9(C) so after activation of the receptor and dimerization of the two domains of dCas9, NLS will direct the (dCas9-VP64-GAL4- UAS Trans CMV enhancer) complex into the nucleus. | ||
+ | <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style=" max-width:850px; | ||
+ | width:75%; | ||
+ | height:auto; | ||
+ | position: relative; | ||
+ | top: 50%; | ||
+ | left: 35%; | ||
+ | transform: translate( -50%); | ||
+ | padding-bottom:25px; | ||
+ | padding-top:25px; | ||
+ | "src="https://static.igem.wiki/teams/5036/parts/nls-attia.png | ||
+ | "> | ||
+ | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
+ | lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of nuclear localization signal (NLS) which is attached to dCas9(c) in dCas9(C)-TF-synVEGFR1 receptor's first chain. | ||
+ | . </span></p></div></html> | ||
+ | ==Experimental Characterization== | ||
+ | we digested dCas9(C)_NLS-Syn-VEGFR-1 grafetd with NLS and performed gel electrophoresis to detect the digested fragment. | ||
+ | <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style=" max-width:850px; | ||
+ | width:75%; | ||
+ | height:auto; | ||
+ | position: relative; | ||
+ | top: 50%; | ||
+ | left: 45%; | ||
+ | transform: translate( -50%); | ||
+ | padding-bottom:25px; | ||
+ | padding-top:25px; | ||
+ | "src="https://static.igem.wiki/teams/5036/lab/sos.png | ||
+ | "> | ||
+ | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
+ | lang=EN style='font-size:11.0pt;line-height:115%'>This figure illustrates the digested dCas9(C)_NLS-Syn-VEGFR-1 prior to insert ligation | ||
+ | . </span></p></div></html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 13:05, 2 October 2024
NLS
Part Description
It is a nuclear localization signal which is a short sequence of amino acids found in certain proteins that acts as a zip code, directing those proteins to the nucleus of a cell. In our model it is attached to dCas9(C) in the first chain of dCas9(C)-TF-synVEGFR1 receptor.
Usage
This part is attached to dCas9(C) so after activation of the receptor and dimerization of the two domains of dCas9, NLS will direct the (dCas9-VP64-GAL4- UAS Trans CMV enhancer) complex into the nucleus.
this figure illustrates the structure of nuclear localization signal (NLS) which is attached to dCas9(c) in dCas9(C)-TF-synVEGFR1 receptor's first chain. .
Experimental Characterization
we digested dCas9(C)_NLS-Syn-VEGFR-1 grafetd with NLS and performed gel electrophoresis to detect the digested fragment.
This figure illustrates the digested dCas9(C)_NLS-Syn-VEGFR-1 prior to insert ligation .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]