Difference between revisions of "Part:BBa K5117000"
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<partinfo>BBa_K5117000 short</partinfo> | <partinfo>BBa_K5117000 short</partinfo> | ||
− | ribosome binding site of Bacillus subtilis (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer | + | This part consists of the ribosome binding site of <i>Bacillus subtilis</i> (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer. |
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+ | BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page). | ||
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+ | <b>Biosafety level:</b> S1 | ||
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+ | <b>Target organism:</b> <i>Bacillus subtilis</i> | ||
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+ | <b>Main purpose of use:</b> Gene expression and protein production using the host <i>B. subtilis</i> | ||
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===References=== | ===References=== | ||
− | Vellanoweth R. L. & Rabinowitz J. C. (1992): The influence of ribosome‐binding‐site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Molecular microbiology 6(9), 1105-1114. | + | Vellanoweth R. L. & Rabinowitz J. C. (1992): The influence of ribosome‐binding‐site elements on translational efficiency in <i>Bacillus subtilis</i> and <i>Escherichia coli in vivo</i>. Molecular microbiology 6(9), 1105-1114. https://doi.org/10.1111/j.1365-2958.1992.tb01548.x |
Latest revision as of 23:35, 1 October 2024
BsRBS
This part consists of the ribosome binding site of Bacillus subtilis (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer.
BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Gene expression and protein production using the host B. subtilis
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Vellanoweth R. L. & Rabinowitz J. C. (1992): The influence of ribosome‐binding‐site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Molecular microbiology 6(9), 1105-1114. https://doi.org/10.1111/j.1365-2958.1992.tb01548.x