Difference between revisions of "Part:BBa K5117010:Design"

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===Design Notes===
 
===Design Notes===
−
removal of restriction sites which are used for RFC10 or TypeIIS standards, addition of a signal peptide SPaprE for secretion in Bacillus subtilis, addition of start codon (ATG) and stop codon (TAA)
+
removal of restriction sites which are used for RFC[10] or Type IIS standards,  
  
 +
addition of a signal peptide SP<sub><i>aprE</i></sub> for secretion in <i>Bacillus subtilis</i>,
  
 +
addition of start codon (ATG) and stop codon (TAA)
  
 
===Source===
 
===Source===
−
 
+
<i>Bacterium HR29</i>
−
Bacterium HR29
+
−
 
+
−
===References===
+
−
Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715.
+

Latest revision as of 19:56, 29 September 2024


BhrPET


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

addition of a signal peptide SPaprE for secretion in Bacillus subtilis,

addition of start codon (ATG) and stop codon (TAA)

Source

Bacterium HR29