Difference between revisions of "Part:BBa K5117007:Design"

 
 
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===Design Notes===
 
===Design Notes===
removal of restriction sites which are used for RFC10 or TypeIIS standards
+
removal of restriction sites which are used for RFC[10] or Type IIS standards,
 
+
  
 +
exchange of start codon (GTG > ATG) for usage in <i>Bacillus subtilis</i> as host organism
  
 
===Source===
 
===Source===
 
+
<i>Bacillus halodurans</i>
Bacillus halodurans
+
 
+
===References===
+

Latest revision as of 23:53, 1 October 2024


BhBglA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 564
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC[10] or Type IIS standards,

exchange of start codon (GTG > ATG) for usage in Bacillus subtilis as host organism

Source

Bacillus halodurans