Difference between revisions of "Part:BBa K5049000"

 
 
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GTH1 (Glucose transporter high affinity 1) gene promoter, a member of the GAP (glyceraldehyde-3-phosphate dehydrogenase) promoter family, offers a more manageable and safer alternative. Although it functions as a constitutive promoter, its activity is finely tuned by glucose levels—it is repressed by excess glycerol and fully induced by low glucose concentrations, specifically at 0.05 g/L (0.005%). This unique regulation allows for precise control over protein expression, making GTH1 promoter particularly suitable for our purpose and desire with high-level expression, without the safety concerns associated with methanol.
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We selected Pichia pastoris as our host organism to develop animal feed additives. In our laboratory setup, we utilized the GTH1 promoter to drive the expression of xylanase fusion proteins, comparing the functionalities of two anchor proteins, GCW61 and Pir1, within the yeast surface display system.
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Latest revision as of 03:39, 10 September 2024


GTH1 Promoter

GTH1 (Glucose transporter high affinity 1) gene promoter, a member of the GAP (glyceraldehyde-3-phosphate dehydrogenase) promoter family, offers a more manageable and safer alternative. Although it functions as a constitutive promoter, its activity is finely tuned by glucose levels—it is repressed by excess glycerol and fully induced by low glucose concentrations, specifically at 0.05 g/L (0.005%). This unique regulation allows for precise control over protein expression, making GTH1 promoter particularly suitable for our purpose and desire with high-level expression, without the safety concerns associated with methanol.

We selected Pichia pastoris as our host organism to develop animal feed additives. In our laboratory setup, we utilized the GTH1 promoter to drive the expression of xylanase fusion proteins, comparing the functionalities of two anchor proteins, GCW61 and Pir1, within the yeast surface display system.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]