Difference between revisions of "Part:BBa K5387000:Design"
 
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===Design Notes===
 
===Design Notes===
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The sequence has been optimized for expression in E. coli.  
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The sequence has been optimized for expression in ''E. coli''.  
  
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Primers for cloning into pET28a(+) between restriction sites NdeI and EcoRI:  
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The vector used was pET-28a(+), which His-ag we utilized when expressing our protein.
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Primers for cloning into pET-28a(+) between restriction sites NdeI and EcoRI:  
 
: fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'  
 
: fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'  
 
: rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'
 
: rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'
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===Source===
 
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This gene is expressed in humans KÄLLA
 
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==References===
 

Latest revision as of 10:31, 29 September 2024

Design Notes

The sequence has been optimized for expression in E. coli.

The vector used was pET-28a(+), which His-ag we utilized when expressing our protein.

Primers for cloning into pET-28a(+) between restriction sites NdeI and EcoRI:

fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'