Difference between revisions of "Part:BBa K5043002"

Latest revision as of 09:35, 30 September 2024

nidB from M. vanbaalenii Pyr-1

nidB forms together with nidA, phtAc and phtAd a pyrene dioxygenase enzyme complex. This complex catalyzes the oxidation of pyrene to cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.

This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrading bacteria. It was also reported that nidAB is able to oxidize other high molecular weight polycyclic aromatic hydrocarbons. [1]

[1] O. Kweon, S.-J. Kim, J. P. Freeman, J. Song, S. Baek, and C. E. Cerniglia, "Substrate specificity and structural characteristics of the novel Rieske nonheme iron aromatic ring-hydroxylating oxygenases NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1," mBio, vol. 1, no. 2, 2010, doi: 10.1128/mBio.00135-10

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 395
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 395
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 395
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 395
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5043002 short</partinfo>
-nidB forms together with nidA, phtAc and phtAd pyrene dioxygenase enzyme complex. This complex catalyzes the oxydation of pyrene to Cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.
+nidB forms together with nidA, phtAc and phtAd a pyrene dioxygenase enzyme complex. This complex catalyzes the oxidation of pyrene to cis-4,5-Dihydro-4,5-dihydroxypyrene. This is the first step in biodegradation of pyrene.
+This part was derived from <i> Mycobacterium vanbaalenii </i> Pyr-1 which is a known pyrene degrading bacteria. It was also reported that nidAB is able to oxidize other high molecular weight polycyclic aromatic hydrocarbons. [1]
+[1] O. Kweon, S.-J. Kim, J. P. Freeman, J. Song, S. Baek, and C. E. Cerniglia, "Substrate specificity and structural characteristics of the novel Rieske nonheme iron aromatic ring-hydroxylating oxygenases NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1," mBio, vol. 1, no. 2, 2010, doi: 10.1128/mBio.00135-10
 <!-- Add more about the biology of this part here