Difference between revisions of "Part:BBa K5071009"

 
 
Line 1: Line 1:
  
−
__NOTOC__
 
 
<partinfo>BBa_K5071009 short</partinfo>
 
<partinfo>BBa_K5071009 short</partinfo>
  
−
BGCII-4
 
  
−
<!-- Add more about the biology of this part here
+
 
−
===Usage and Biology===
+
 
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5071009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5071009 SequenceAndFeatures</partinfo>
 +
<!DOCTYPE html>
 +
<html lang="en">
 +
<head>
 +
    <meta charset="UTF-8">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
 +
    <title>BBa_K5071009 (BGCII-4)</title>
 +
    <style>
 +
        img {
 +
            max-width: 80%;
 +
            height: auto;
 +
        }
 +
        .caption {
 +
            text-align: center;
 +
            font-size: 0.9em;
 +
            margin-top: 5px;
 +
            margin-bottom: 20px;
 +
        }
 +
        table {
 +
            width: 100%;
 +
            border-collapse: collapse;
 +
            margin-top: 20px;
 +
            margin-bottom: 20px;
 +
        }
 +
        th, td {
 +
            border: 1px solid #ddd;
 +
            padding: 8px;
 +
            text-align: center;
 +
        }
 +
        th {
 +
            background-color: #f2f2f2;
 +
        }
 +
    </style>
 +
</head>
 +
<body>
 +
    <h2>Composite part BBa_K5071009 (BGCII-4)</h2>
  
 +
    <h3>Name: BGCII-4</h3>
 +
    <p><strong>Base Pairs:</strong> 150 bp</p>
 +
    <p><strong>Origin:</strong> Paceibacterales</p>
  
−
<!-- Uncomment this to enable Functional Parameter display
+
    <h3>Usage and Biology</h3>
−
===Functional Parameters===
+
    <p>
−
<partinfo>BBa_K5071009 parameters</partinfo>
+
        BGCII-4 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.
−
<!-- -->
+
    </p>
 +
 
 +
    <h3>Cultivation</h3>
 +
    <p>
 +
        We used PCR to amplify the BGCII-4 gene, with a length of 150 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pACYCDuet-BGCII-gene143.
 +
    </p>
 +
 
 +
    <div style="text-align:center;">
 +
        <img src="https://static.igem.wiki/teams/5071/bba-k5071009/1.jpg" alt="Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene143">
 +
        <div class="caption">Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene143</div>
 +
    </div>
 +
 
 +
</body>
 +
</html>

Latest revision as of 04:51, 30 September 2024

BGCII-4



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

<!DOCTYPE html> BBa_K5071009 (BGCII-4)

Composite part BBa_K5071009 (BGCII-4)

Name: BGCII-4

Base Pairs: 150 bp

Origin: Paceibacterales

Usage and Biology

BGCII-4 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.

Cultivation

We used PCR to amplify the BGCII-4 gene, with a length of 150 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pACYCDuet-BGCII-gene143.

Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene143
Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene143