Difference between revisions of "Part:BBa K5291007:Design"
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===Design Notes===
 
===Design Notes===
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We use it to improve the efficiency of carbon delivery between the two engineered bacteria to avoid the harm of CO2 release. And we choose PAO102 because it natively exists in Pseudomonas aeruginosa PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered P.aeruginosa.
+
Given that CO<sub>2</sub> is hard to trasnport in the biofilm formed by the two kinds of engineered bacteria, we use <i>PAO102</i> to improve the efficiency of carbon delivery to avoid the harm of CO<sub>2</sub> release. And we choose this gene because it natively exists in <i>Pseudomonas aeruginosa</i> PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered <i>P.aeruginosa</i>.
  
 
===Source===
 
===Source===
  
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Pseudomonas aeruginosa PAO1
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<i>Pseudomonas aeruginosa</i> PAO1
  
 
===References===
 
===References===
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[1]Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in  survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.
+
[1]Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in  survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.<br>
 
[2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.
 
[2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.

Latest revision as of 03:33, 28 September 2024


PAO102


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 153
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Given that CO2 is hard to trasnport in the biofilm formed by the two kinds of engineered bacteria, we use PAO102 to improve the efficiency of carbon delivery to avoid the harm of CO2 release. And we choose this gene because it natively exists in Pseudomonas aeruginosa PAO1 as well as its broad pH adjustion, which ensures its normal expression in the engineered P.aeruginosa.

Source

Pseudomonas aeruginosa PAO1

References

[1]Lotlikar, S.R., et al., Three functional beta-carbonic anhydrases in Pseudomonas aeruginosa PAO1: role in survival in ambient air. Microbiology (Reading), 2013. 159(Pt 8): p. 1748-1759.
[2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.