Difference between revisions of "Part:BBa K5143001"
 
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         <figure>
 
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             <img src="https://static.igem.wiki/teams/5143/bba-k5143001-masp1.png" width="400" alt="MaSp1">
 
             <img src="https://static.igem.wiki/teams/5143/bba-k5143001-masp1.png" width="400" alt="MaSp1">
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             <figcaption>Figure 1: MaSp1 Gene</figcaption>
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             <figcaption><i><u>Figure 1:</u> MaSp1 Gene</i></figcaption>
 
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         <figure>
 
         <figure>
 
             <img src="https://static.igem.wiki/teams/5143/bba-k5143002-spider-silk.jpg" width="200" alt="Spider Silk">
 
             <img src="https://static.igem.wiki/teams/5143/bba-k5143002-spider-silk.jpg" width="200" alt="Spider Silk">
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             <figcaption>Figure 2: Spider Silk</figcaption>
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             <figcaption><i><u>Figure 2:</u> Spider Silk</i></figcaption>
 
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<br>
 
     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
     <p>
 
     <p>
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         The MaSp1 gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a barnacle ciment protein as a bioglue, to improve its adhesive properties: <a href="https://parts.igem.org/Part:BBa_K5143003" target="_blank">BBa_K5143003</a>
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         The MaSp1 gene was synthesised and its nucleotide sequence optimised for synthesis and expression in <I> Saccharomyces cerevisiae</I>. This protein is used in fusion with a barnacle ciment protein as a bioglue, to improve its adhesive properties: <a href="https://parts.igem.org/Part:BBa_K5143003" target="_blank">BBa_K5143003</a>
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    </p>
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    <h1>References</h1>
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    <p>
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        Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<h1>Sequence and Features</h1>
 
<partinfo>BBa_K5143001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5143001 SequenceAndFeatures</partinfo>
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<br>
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<h1>References</h1>
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    <p>
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        Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). <br>
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<br>
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Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. <br>
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<br>
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A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.
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    </p>
  
 
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Latest revision as of 14:10, 1 October 2024


MaSp1 : a spider silk protein used as a bioglue, optimised for Saccharomyces cerevisiae

Protein Description

Description

MaSp1 is a spider silk protein that can be used as a bioglue. The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m².

MaSp1
Figure 1: MaSp1 Gene
Spider Silk
Figure 2: Spider Silk

Construction

The MaSp1 gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a barnacle ciment protein as a bioglue, to improve its adhesive properties: BBa_K5143003

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 191
    Illegal PstI site found at 494
    Illegal PstI site found at 587
    Illegal PstI site found at 593
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022).

Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118.

A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.