Difference between revisions of "Part:BBa K5143015"

 
 
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    <title>Protein Description</title>
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    <h1>Description</h1>
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        Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (Part:BBa_K4081994). The excitation wavelength is 516nm and the emission wavelength is 530nm.
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        <img src="https://static.igem.wiki/teams/5143/bba-k5143015-venus.png" width="800" alt="Venus"> <!-- Taille de l'image augmentée -->
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        <div class="image-caption">Venus Yellow Fluorescent Protein used as a transcriptional reporter.</div>
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    <h1>Construction</h1>
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      The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (Part:BBa_K5143009) in 5'. See the composite part here:
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    <h1>References</h1>
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1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).<br>
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2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
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Latest revision as of 19:44, 31 July 2024


Venus : reporter Yellow Fluorescent Protein

Protein Description

Description

Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (Part:BBa_K4081994). The excitation wavelength is 516nm and the emission wavelength is 530nm.

Venus
Venus Yellow Fluorescent Protein used as a transcriptional reporter.

Construction

The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (Part:BBa_K5143009) in 5'. See the composite part here:

References

1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).
2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]