Difference between revisions of "Part:BBa K5143010"
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<title>Protein Description</title> | <title>Protein Description</title> | ||
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<body> | <body> | ||
<h1>Description</h1> | <h1>Description</h1> | ||
<p> | <p> | ||
− | + | The α-mating factor is a peptide hormone produced by the yeast <i>Saccharomyces cerevisiae</i>, which is crucial for the mating process of these yeast cells. Here are the key details about the α-mating factor: | |
</p> | </p> | ||
− | <img src="" width=" | + | <ul> |
+ | <li><strong>Function:</strong> It is involved in the mating process of <i>S. cerevisiae</i> by being secreted by α-type yeast cells to attract a-type cells, facilitating the mating interaction.</li> | ||
+ | <li><strong>Production:</strong> Synthesized as a pre-protein that includes a signal peptide and the mature α-mating factor peptide.</li> | ||
+ | <li><strong>Structure and Processing:</strong> | ||
+ | <ul> | ||
+ | <li><strong>Pre-Pro-α-Mating Factor:</strong> Initially synthesized with a signal peptide (pre-region) that directs the nascent protein to the secretory pathway.</li> | ||
+ | <li><strong>Mature Peptide:</strong> After cleavage of the signal peptide, the remaining peptide is processed into the active α-mating factor.</li> | ||
+ | <li><strong>Processing:</strong> Includes cleavage of the signal peptide and pro-region to yield the mature peptide.</li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><strong>Secretion:</strong> | ||
+ | <ul> | ||
+ | <li><strong>Signal Peptide Function:</strong> Directs the protein to the endoplasmic reticulum (ER), where it is cleaved for passage through the secretory pathway.</li> | ||
+ | <li><strong>Mature Peptide Secretion:</strong> Transported through the Golgi apparatus and secreted into the extracellular environment.</li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><strong>Applications in Biotechnology:</strong> | ||
+ | <ul> | ||
+ | <li><strong>Protein Secretion:</strong> The α-mating factor's signal peptide is used to enhance the secretion of various proteins in yeast by attaching it to a protein of interest.</li> | ||
+ | <li><strong>Engineering Yeast Strains:</strong> Yeast strains can be engineered for efficient production of heterologous proteins by incorporating the α-mating factor signal peptide into their expression constructs.</li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | <img src="https://static.igem.wiki/teams/5143/bba-k5143009-sc-protein-a.png" width="600" alt="α-mating factor" class="center-image"> | ||
<h1>Construction</h1> | <h1>Construction</h1> | ||
<p> | <p> | ||
− | + | he Alpha-Factor gene was synthesised and its nucleotide sequence optimised for DNA synthesis and expression in <i>Saccharomyces cerevisiae</i>. | |
− | + | The gene exists in 2 versions: which limits homologous recombination in the <i>Saccharomyces cerevisiae</i> genome. The second version can be found here : <a href="https://parts.igem.org/Part:BBa_K5143010" target="_blank">BBa_K5143010</a> | |
+ | <br>These two versions of the alpha-factor can be found in the following composite part: </p> | ||
<h1>References</h1> | <h1>References</h1> | ||
<p> | <p> | ||
− | + | Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984). | |
+ | Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021). | ||
</p> | </p> | ||
</body> | </body> | ||
</html> | </html> | ||
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Latest revision as of 10:16, 31 July 2024
Alpha-Factor secretion signal V2 optimized for expression in Saccharomyces cerevisiae
Description
The α-mating factor is a peptide hormone produced by the yeast Saccharomyces cerevisiae, which is crucial for the mating process of these yeast cells. Here are the key details about the α-mating factor:
- Function: It is involved in the mating process of S. cerevisiae by being secreted by α-type yeast cells to attract a-type cells, facilitating the mating interaction.
- Production: Synthesized as a pre-protein that includes a signal peptide and the mature α-mating factor peptide.
- Structure and Processing:
- Pre-Pro-α-Mating Factor: Initially synthesized with a signal peptide (pre-region) that directs the nascent protein to the secretory pathway.
- Mature Peptide: After cleavage of the signal peptide, the remaining peptide is processed into the active α-mating factor.
- Processing: Includes cleavage of the signal peptide and pro-region to yield the mature peptide.
- Secretion:
- Signal Peptide Function: Directs the protein to the endoplasmic reticulum (ER), where it is cleaved for passage through the secretory pathway.
- Mature Peptide Secretion: Transported through the Golgi apparatus and secreted into the extracellular environment.
- Applications in Biotechnology:
- Protein Secretion: The α-mating factor's signal peptide is used to enhance the secretion of various proteins in yeast by attaching it to a protein of interest.
- Engineering Yeast Strains: Yeast strains can be engineered for efficient production of heterologous proteins by incorporating the α-mating factor signal peptide into their expression constructs.
![α-mating factor](https://static.igem.wiki/teams/5143/bba-k5143009-sc-protein-a.png)
Construction
he Alpha-Factor gene was synthesised and its nucleotide sequence optimised for DNA synthesis and expression in Saccharomyces cerevisiae.
The gene exists in 2 versions: which limits homologous recombination in the Saccharomyces cerevisiae genome. The second version can be found here : BBa_K5143010
These two versions of the alpha-factor can be found in the following composite part:
References
Brake, A. J. et al. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 81, 4642–4646 (1984). Aza, P., Molpeceres, G., de Salas, F. & Camarero, S. Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast. Cell Mol Life Sci 78, 3691–3707 (2021).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
Illegal NheI site found at 211 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]