Difference between revisions of "Part:BBa K5143001"
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<title>Protein Description</title> | <title>Protein Description</title> | ||
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<h1>Description</h1> | <h1>Description</h1> | ||
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| − | + | MaSp1 is a spider silk protein that can be used as a bioglue. | |
| − | The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m². </p> | + | The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m². |
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| + | <img src="https://static.igem.wiki/teams/5143/bba-k5143001-masp1.png" width="400" alt="MaSp1"> | ||
| + | <figcaption>Figure 1: MaSp1 Gene</figcaption> | ||
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| + | <img src="https://static.igem.wiki/teams/5143/bba-k5143002-spider-silk.jpg" width="200" alt="Spider Silk"> | ||
| + | <figcaption>Figure 2: Spider Silk</figcaption> | ||
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<h1>Construction</h1> | <h1>Construction</h1> | ||
<p> | <p> | ||
| − | + | The MaSp1 gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a barnacle ciment protein as a bioglue, to improve its adhesive properties: <a href="https://parts.igem.org/Part:BBa_K5143003" target="_blank">BBa_K5143003</a> | |
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</p> | </p> | ||
<h1>References</h1> | <h1>References</h1> | ||
<p> | <p> | ||
| − | Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). | + | Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/. |
| − | Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. | + | </p> |
| − | A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/. | + | |
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Latest revision as of 16:04, 30 July 2024
MaSp1 : a spider silk protein used as a bioglue, optimised for Saccharomyces cerevisiae
Description
MaSp1 is a spider silk protein that can be used as a bioglue. The dragline protein MaSp1 containing numerous alanine and glycine residues tends to form considerable β-sheet and random coil secondary structure, which account for the crystalline fraction of cobwebs and confer inherently remarkable mechanical properties. The adhesion strenght of MaSp1 : 22.7 mJ/m².
Construction
The MaSp1 gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with a barnacle ciment protein as a bioglue, to improve its adhesive properties: BBa_K5143003
References
Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 22
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 191
Illegal PstI site found at 494
Illegal PstI site found at 587
Illegal PstI site found at 593 - 1000COMPATIBLE WITH RFC[1000]