Difference between revisions of "Part:BBa K5143002"

 
 
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Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion.  
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    <title>Protein Description</title>
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    <h1>Description</h1>
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        Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion. Adhesion strength: 2.2 mJ/m². See <a href="https://parts.igem.org/Part:BBa_K3089008" target="_blank">BBa_K3089008</a> which provided the inspiration for the discovery of this sticky protein.
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143002-cp19k.png" width="400" alt="NOM PHOTO">
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            <figcaption>Figure 1: Cp19k Gene</figcaption>
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            <img src="https://static.igem.wiki/teams/5143/bba-k5143002-barnacle.jpg" width="200" alt="Barnacle">
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            <figcaption>Figure 2: Barnacle on a rock surface</figcaption>
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    <h1>Construction</h1>
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        The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>. This protein is used in fusion with spider silk protein as a bioglue, to improve its adhesive properties: <a href="https://parts.igem.org/Part:BBa_K5143003" target="_blank">BBa_K5143003</a>
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    <h1>References</h1>
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        Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:17, 30 July 2024


Cp19k : a barnacle cement protein used as a bioglue, optimised for Saccharomyces cerevisiae

Protein Description

Description

Barnacle, a marine organism, easily stick to underwater matrices through the secretion of various proteins and forming cement complexes. The protein complex for barnacle, Cp19k plays a key role in interfacial adhesion. Adhesion strength: 2.2 mJ/m². See BBa_K3089008 which provided the inspiration for the discovery of this sticky protein.

NOM PHOTO
Figure 1: Cp19k Gene
Barnacle
Figure 2: Barnacle on a rock surface

Construction

The Cp19k gene was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. This protein is used in fusion with spider silk protein as a bioglue, to improve its adhesive properties: BBa_K5143003

References

Malay, A. D., Craig, H. C., Chen, J., Oktaviani, N. A. & Numata, K. Complexity of Spider Dragline Silk. Biomacromolecules 23, 1827–1840 (2022). Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk | PNAS. https://www-pnas-org.docelec.univ-lyon1.fr/doi/full/10.1073/pnas.2107065118. A bioinspired synthetic fused protein adhesive from barnacle cement and spider dragline for potential biomedical materials - PubMed. https://pubmed.ncbi.nlm.nih.gov/37776922/.





Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]