Difference between revisions of "Part:BBa K5226000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation. Mutate the base pairs at 678bp, 1320bp and 1326bp of AM1-ftfl from g/c to a/t. | |
− | + | ||
===Source=== | ===Source=== | ||
− | Synthetic(BGI). Sequence source is from Methylobacterium extorquens AM1, found through NCBI (https://www.ncbi.nlm.nih.gov/nuccore/CP001510. | + | Synthetic(BGI). Sequence source is from Methylobacterium extorquens AM1, found through NCBI (https://www.ncbi.nlm.nih.gov/nuccore/CP001510.1?from=340743&to=342416) |
===References=== | ===References=== |
Latest revision as of 03:51, 21 August 2024
AM1-ftfl
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 379
Illegal AgeI site found at 693
Illegal AgeI site found at 721
Illegal AgeI site found at 859 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Although this part complies with one of the RFC 10 or Type IIS standards, it is inconsistent with the standards upheld by other basic parts. As a result, the composite component formed by their combination has illegal sites, necessitating a synonymous mutation. Mutate the base pairs at 678bp, 1320bp and 1326bp of AM1-ftfl from g/c to a/t.
Source
Synthetic(BGI). Sequence source is from Methylobacterium extorquens AM1, found through NCBI (https://www.ncbi.nlm.nih.gov/nuccore/CP001510.1?from=340743&to=342416)