Difference between revisions of "Part:BBa K4620020"

 
 
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Arb2BURP from Coffea arabica is a split cyclase - naturally expressed separately from its peptide substrate and therefore had greater potential to cyclase various peptide substrates. We cloned Arb2BURP into MBP-pEXP expression vector. The protein was expressed in 2TY medium overnight at 18 ⁰C, purified using Ni-NTA chromatography in 8 M urea buffer, and refolded at 4 ⁰C overnight. Refolded protein was applied to 75 pg SEC column.
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Latest revision as of 15:57, 12 October 2023


Arb2BURP

Arb2BURP from Coffea arabica is a split cyclase - naturally expressed separately from its peptide substrate and therefore had greater potential to cyclase various peptide substrates. We cloned Arb2BURP into MBP-pEXP expression vector. The protein was expressed in 2TY medium overnight at 18 ⁰C, purified using Ni-NTA chromatography in 8 M urea buffer, and refolded at 4 ⁰C overnight. Refolded protein was applied to 75 pg SEC column.

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 211
    Illegal PstI site found at 424
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 211
    Illegal PstI site found at 424
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 211
    Illegal PstI site found at 424
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 211
    Illegal PstI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]