Difference between revisions of "Part:BBa K4990013"
 
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As HokB belongs to the Hok/Gef family, it is predicted to be a single-span membrane peptide，which is a single-span membrane peptide with a long, negatively charged C-terminal periplasmic tail and a short, positively charged N terminus extending in the cytoplasm. HokB has the ability to selectively insert into the cellular membrane and aggregate to create pores with a diameter of around 0.64nm, which alters the membrane potential, depolarize the membrane, hinder ATP synthesis, decrease the cell's energy ratio, and lead to ATP leakage,[1] ultimately resulting in cell death.
 
As HokB belongs to the Hok/Gef family, it is predicted to be a single-span membrane peptide，which is a single-span membrane peptide with a long, negatively charged C-terminal periplasmic tail and a short, positively charged N terminus extending in the cytoplasm. HokB has the ability to selectively insert into the cellular membrane and aggregate to create pores with a diameter of around 0.64nm, which alters the membrane potential, depolarize the membrane, hinder ATP synthesis, decrease the cell's energy ratio, and lead to ATP leakage,[1] ultimately resulting in cell death.
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<img src="https://static.igem.wiki/teams/4990/wiki/registry/hokb structure.jpg" width="850" >
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<https://static.igem.wiki/teams/4990/wiki/registry/hokb-2dstructure.jpg" width="850" >
 
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Fig 1.Computational predictions of HokB using the Kyte-Doolittle algorithm and the CBS TMHMM tool.[1]
 
  
  
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REFERENCE
 
REFERENCE
  
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[1]Wilmaerts, D., Bayoumi, M., Dewachter, L., Knapen, W., Mika, J. T., Hofkens, J., Dedecker, P., Maglia, G., Verstraeten, N., & Michiels, J. (2018). The Persistence-Inducing Toxin HokB Forms Dynamic Pores That Cause ATP Leakage. mBio, 9(4), e00744-18. https://doi.org/10.1128/mBio.00744-18
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[1] Wilmaerts, D., Bayoumi, M., Dewachter, L., Knapen, W., Mika, J. T., Hofkens, J., Dedecker, P., Maglia, G., Verstraeten, N., & Michiels, J. (2018). The Persistence-Inducing Toxin HokB Forms Dynamic Pores That Cause ATP Leakage. mBio, 9(4), e00744-18. https://doi.org/10.1128/mBio.00744-18
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 15:55, 12 October 2023


HokB


As HokB belongs to the Hok/Gef family, it is predicted to be a single-span membrane peptide，which is a single-span membrane peptide with a long, negatively charged C-terminal periplasmic tail and a short, positively charged N terminus extending in the cytoplasm. HokB has the ability to selectively insert into the cellular membrane and aggregate to create pores with a diameter of around 0.64nm, which alters the membrane potential, depolarize the membrane, hinder ATP synthesis, decrease the cell's energy ratio, and lead to ATP leakage,[1] ultimately resulting in cell death.





REFERENCE

[1] Wilmaerts, D., Bayoumi, M., Dewachter, L., Knapen, W., Mika, J. T., Hofkens, J., Dedecker, P., Maglia, G., Verstraeten, N., & Michiels, J. (2018). The Persistence-Inducing Toxin HokB Forms Dynamic Pores That Cause ATP Leakage. mBio, 9(4), e00744-18. https://doi.org/10.1128/mBio.00744-18

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]