Difference between revisions of "Part:BBa K4770029"
 
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<partinfo>BBa_K4770029 short</partinfo>
 
<partinfo>BBa_K4770029 short</partinfo>
 +
 
===Description and Biology===
 
===Description and Biology===
−
Level Ms are the assembly of different transcriptional units into the same vector.  
+
Level Ms are the assembly of different transcriptional units into the same vector.
 +
 
All AlgaGenix’s Level M follow the same structure: they contain the HygroR cassette, level 1 for each gene (pCM1:NR, NiR, GS, Cp-GS, IPT or LOG), mVenus cassette, and mCherry cassette.  
 
All AlgaGenix’s Level M follow the same structure: they contain the HygroR cassette, level 1 for each gene (pCM1:NR, NiR, GS, Cp-GS, IPT or LOG), mVenus cassette, and mCherry cassette.  
 +
 
In this case, we are using pCM1:Cp_GS
 
In this case, we are using pCM1:Cp_GS
 +
 
With this vector, we aim to see the effects of overexpressing GS constitutively in the chloroplast and at the same time normalizing the expression level depending on the insertion site of the vector (by measuring the signal of the mCherry cassette), at the same time, we will be able to see the decrease of nitrates concentration fue to higher assimilation thanks to the mVenus cassette signal.  
 
With this vector, we aim to see the effects of overexpressing GS constitutively in the chloroplast and at the same time normalizing the expression level depending on the insertion site of the vector (by measuring the signal of the mCherry cassette), at the same time, we will be able to see the decrease of nitrates concentration fue to higher assimilation thanks to the mVenus cassette signal.  
 +
 
===How can this part be used===
 
===How can this part be used===
 
This Level M is aimed to assembled with HygroR cassette in position 4, Cp-GS Level1 in position 5, mCherry cassette in position 6 and mVenus cassette in position 7.  
 
This Level M is aimed to assembled with HygroR cassette in position 4, Cp-GS Level1 in position 5, mCherry cassette in position 6 and mVenus cassette in position 7.  
 +
 
Should you use this part and need advice on how to use it, please do not hesitate to contact us at algagenix@gmail.com
 
Should you use this part and need advice on how to use it, please do not hesitate to contact us at algagenix@gmail.com
 +
 
===Source of this part===
 
===Source of this part===
 
Position 4: Hygro R cassette BBa_K4770016
 
Position 4: Hygro R cassette BBa_K4770016
 +
 
Position 5: pCM1 Cp-GS BBa_K4770021
 
Position 5: pCM1 Cp-GS BBa_K4770021
 +
 
Position 6: mCherry cassette BBa_K4770017
 
Position 6: mCherry cassette BBa_K4770017
 +
 
Position 7: mVenus cassette BBa_K4770025
 
Position 7: mVenus cassette BBa_K4770025
 +
 
===Design considerations===
 
===Design considerations===
 
AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit.  Each piece has it’s own design considerations that can be looked up on the individual part’s pages.  
 
AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit.  Each piece has it’s own design considerations that can be looked up on the individual part’s pages.  
  
 
<partinfo>BBa_K4770029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4770029 SequenceAndFeatures</partinfo>

Latest revision as of 12:20, 12 October 2023

Cp_GS_Chloroplast_Glutamate_Synthetase_Level_M

Description and Biology

Level Ms are the assembly of different transcriptional units into the same vector.

All AlgaGenix’s Level M follow the same structure: they contain the HygroR cassette, level 1 for each gene (pCM1:NR, NiR, GS, Cp-GS, IPT or LOG), mVenus cassette, and mCherry cassette.

In this case, we are using pCM1:Cp_GS

With this vector, we aim to see the effects of overexpressing GS constitutively in the chloroplast and at the same time normalizing the expression level depending on the insertion site of the vector (by measuring the signal of the mCherry cassette), at the same time, we will be able to see the decrease of nitrates concentration fue to higher assimilation thanks to the mVenus cassette signal.

How can this part be used

This Level M is aimed to assembled with HygroR cassette in position 4, Cp-GS Level1 in position 5, mCherry cassette in position 6 and mVenus cassette in position 7.

Should you use this part and need advice on how to use it, please do not hesitate to contact us at algagenix@gmail.com

Source of this part

Position 4: Hygro R cassette BBa_K4770016

Position 5: pCM1 Cp-GS BBa_K4770021

Position 6: mCherry cassette BBa_K4770017

Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4830
    Illegal PstI site found at 7168
    Illegal PstI site found at 8686
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4830
    Illegal PstI site found at 7168
    Illegal PstI site found at 8686
    Illegal NotI site found at 8422
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4830
    Illegal BglII site found at 5168
    Illegal BamHI site found at 4474
    Illegal XhoI site found at 4686
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4830
    Illegal PstI site found at 7168
    Illegal PstI site found at 8686
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4830
    Illegal PstI site found at 7168
    Illegal PstI site found at 8686
    Illegal NgoMIV site found at 1265
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 4032
    Illegal NgoMIV site found at 4984
    Illegal NgoMIV site found at 9163
  • 1000
    COMPATIBLE WITH RFC[1000]