Difference between revisions of "Part:BBa K4770026"

Latest revision as of 12:04, 12 October 2023

NR_Nitrate_Reductase_Level_M

Level Ms are the assembly of different transcriptional units into the same vector. All AlgaGenix’s Level M follow the same structure: they contain the HygroR cassette, level 1 for each gene (pCM1:NR, NiR, GS, Cp-GS, IPT or LOG), mVenus cassette, and mCherry cassette. In this case, we are using pCM1:NR With this vector, we aim to see the effects of overexpressing NR constitutively and at the same time normalizing the expression level depending on the insertion site of the vector (by measuring the signal of the mCherry cassette), at the same time, we will be able to see the decrease of nitrates concentration fue to higher assimilation thanks to the mVenus cassette signal.

How can this part be used

This Level M is aimed to be assembled with HygroR cassette in position 4, NR Level1 in position 5, mCherry cassette in position 6, and mVenus cassette in position 7. Should you use this part and need advice on how to use it, please do not hesitate to contact us at algagenix@gmail.com

Source of this part

Position 4: Hygro R cassette BBa_K4770016

Position 5: pCM1 NR BBa_K4770018

Position 6: mCherry cassette BBa_K4770017

Position 7: mVenus cassette BBa_K4770025

Design considerations

AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit. Each piece has it’s own design considerations that can be looked up on the individual part’s pages.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 3882
Illegal PstI site found at 5146
Illegal PstI site found at 8289
Illegal PstI site found at 9815
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 5726
Illegal PstI site found at 3882
Illegal PstI site found at 5146
Illegal PstI site found at 8289
Illegal PstI site found at 9815
Illegal NotI site found at 9551
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 6281
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 3882
Illegal PstI site found at 5146
Illegal PstI site found at 8289
Illegal PstI site found at 9815
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 3882
Illegal PstI site found at 5146
Illegal PstI site found at 8289
Illegal PstI site found at 9815
Illegal NgoMIV site found at 1265
Illegal NgoMIV site found at 1447
Illegal NgoMIV site found at 3771
Illegal NgoMIV site found at 4365
Illegal NgoMIV site found at 4980
Illegal NgoMIV site found at 6097
Illegal NgoMIV site found at 10292
Illegal AgeI site found at 3543
Illegal AgeI site found at 5856
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 <partinfo>BBa_K4770026 short</partinfo>
 ===Description and Biology===
 Level Ms are the assembly of different transcriptional units into the same vector.
@@ Line 13: / Line 13: @@
 ===Source of this part===
 Position 4: Hygro R cassette BBa_K4770016
 Position 5: pCM1 NR  BBa_K4770018
 Position 6: mCherry cassette BBa_K4770017
 Position 7: mVenus cassette BBa_K4770025
 ===Design considerations===
 AlgaGenix’s Level Ms are designed to have the same structure and not having to build different pieces with different positions for each transcriptional unit.  Each piece has it’s own design considerations that can be looked up on the individual part’s pages.
+===Sequence and Features===
 <partinfo>BBa_K4770026 SequenceAndFeatures</partinfo>