Difference between revisions of "Part:BBa K283022:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K283022 short</partinfo>
 
<partinfo>BBa_K283022 short</partinfo>
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The BBa_K283016 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the ''cheZ'' encoding gene was inserted between XbaI and SpeI.
  
 
<partinfo>BBa_K283022 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K283022 SequenceAndFeatures</partinfo>
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===Source===
 
===Source===
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This part is composited from BBa_R0010, BBa_B0034 and BBa_K094100
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The chez coding region is self-designed
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The rbs and the pLac promomter are from the registry
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===References===
 
===References===

Latest revision as of 17:05, 21 October 2009

pLacI-rbs-cheZ

The BBa_K283016 has been made from this composite. The backbone of this biobrick is based on BBa_J61003. First, we changed the pTet-rbs into pLacI-rbs. Then the cheZ encoding gene was inserted between XbaI and SpeI.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 872
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This part is composited from BBa_R0010, BBa_B0034 and BBa_K094100

References