Difference between revisions of "Part:BBa K265011"
 
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<partinfo>BBa_K265011 short</partinfo>
 
<partinfo>BBa_K265011 short</partinfo>
  
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This part is a fusion of a modified OmpA with a signal sequence to allow cleavage when a protein is fused. OmpA was modified by replacing the normal restriction sites with sites for RFC12 fusion format.
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Since OmpA is believed to function similarly to INPNC and INPNC believed to function better when it is used with the right signal sequence (BBa_K265002)(1), we have decided to test and see if OmpA's ability to secret increases when it is used by a signal sequence.
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An OmpA/protein fusion normally allows display of proteins on the cell surface by way of a linker. The addition of this signal sequence allows secretion by cleavage from the OmpA linker after display.
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We have submitted this part to the part registry.
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For more information on OmpA go to: BBa_K103006 
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This part is authored by Richard Hauser, Tim Fenton, Willis Wong, Lydia Kwon, Jimmy Huang, and Pantea Gheibi.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:03, 22 October 2009

OmpA with proteolytic cleavage tag

Since OmpA is believed to function similarly to INPNC and INPNC believed to function better when it is used with the right signal sequence (BBa_K265002)(1), we have decided to test and see if OmpA's ability to secret increases when it is used by a signal sequence. We have submitted this part to the part registry. For more information on OmpA go to: BBa_K103006

This part is authored by Richard Hauser, Tim Fenton, Willis Wong, Lydia Kwon, Jimmy Huang, and Pantea Gheibi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]