Difference between revisions of "Part:BBa K4814012"
 
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<partinfo>BBa_K4814012 short</partinfo>
 
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To attempt to improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates.  
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To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. However, our results indicated that Strong RBS did not improve performance of the reporter.
 
Sequence: TTTAAGAGGGGGCTATACAT
 
Sequence: TTTAAGAGGGGGCTATACAT
  
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Click here for the experiment data: <html><a href="https://parts.igem.org/Part:BBa_K4814013">RecA(K3)-Strong-eGFP BBa_K4814013</a></html>
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-uvb.png alt=""style="width:400px;height:auto;">
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-h2o2.png alt=""style="width:400px;height:auto;">
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<p>RBS test of UVB and H2O2 FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p>
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-na.png alt=""style="width:400px;height:auto;">
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/rbs-ap-1.png alt=""style="width:400px;height:auto;">
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<p>RBS test of Nalidixic acid and Aspartame FL over OD600 of RecA(K3)-B0034-eGFP, RecA(K3)-B0032-eGFP, and RecA(K3)-Strong-eGFP.</p>
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References:
 
References:

Latest revision as of 09:57, 12 October 2023


Strong RBS

To improve the RecA (K3) - B0034 - EGFP bioreporter we designed to test RecA(K3) (BBa_K3020001) promoter, we replaced the RBS with B0032 and a new RBS invented by Zhang, M. et al (2022). The researchers developed a machine learning model to predict the translation initiation rate of different RBS sequences. Then, the designed sequences were synthesized and experimentally tested for their translation initiation rates. However, our results indicated that Strong RBS did not improve performance of the reporter. Sequence: TTTAAGAGGGGGCTATACAT

Click here for the experiment data: RecA(K3)-Strong-eGFP BBa_K4814013

References:

Zhang, M., Holowko, M. B., Zumpe, H. H., & Ong, C. S. (2022). Machine Learning Guided Batched Design of a Bacterial Ribosome Binding Site. ACS Synthetic Biology, 11(7), 2314-2326. https://doi.org/10.1021/acssynbio.2c00015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]