Difference between revisions of "Part:BBa K4837000"

Latest revision as of 08:41, 12 October 2023

Lignin peroxidase

Optimized the sequence for E. coli expression

This plasmid optimized the sequence from white-rot fungus ( Phanerochaete chrysosporium) for E. coli expression.

The product illustrated below, namely lignin peroxidase (LiP), works together with a DNA duplex and another product from BBa_K4837001 to form a "cascade enzyme system" for PFAs degradation.

LiP is conjugated with an azide-modified DNA oligo, which is illustrated below:

Finally, the "cascade enzyme system" formed when two DNA oligos in the conjugated products bound with each other by complementary base pairing principle, which is illustrated below:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 126
Illegal NheI site found at 1694
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 66
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 260
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2352

@@ Line 5: / Line 5: @@
 Optimized the sequence for E. coli expression
-This plasmid optimized the sequence from white-rot fungus (<i> Phanerochaete chrysosporium</i>) for E. coli expression.
+This plasmid optimized the sequence from white-rot fungus (<i> Phanerochaete chrysosporium</i>) for <i> E. coli </i> expression.
@@ Line 15: / Line 15: @@
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-MnP is conjugated with an azide-modified DNA oligo, which is illustrated below:
+LiP is conjugated with an azide-modified DNA oligo, which is illustrated below:
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-<a href='https://static.igem.wiki/teams/4837/wiki/description-photo-1.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/description-photo-1.png" width="35%"/>
+<a href='https://static.igem.wiki/teams/4837/wiki/description-photo-3.png' target="_blank"><img src="https://static.igem.wiki/teams/4837/wiki/description-photo-3.png" width="35%"/>
 </a>
 </html>