Difference between revisions of "Part:BBa K4683024:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | ===Applications of | + | |
| + | __NOTOC__ | ||
| + | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| + | how you used this part and how it worked out. | ||
| + | |||
| + | ===Applications of BBa K4683024=== | ||
| + | <b> Lambert_GA 2023</b> | ||
| + | <br> | ||
| + | Gel Experimentation | ||
| + | <br> | ||
| + | We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful. | ||
| + | <br> | ||
| + | <html><img src="https://static.igem.wiki/teams/4683/wiki/human-practices/igem-estrogen-label.png" | ||
| + | alt="Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes) | ||
| + | " width="500"></html> | ||
| + | <br> | ||
| + | Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells. | ||
| + | |||
| + | |||
===User Reviews=== | ===User Reviews=== | ||
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| − | <partinfo> | + | <partinfo>BBa_K4683005 AddReview number</partinfo> |
<I>Username</I> | <I>Username</I> | ||
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Latest revision as of 14:00, 12 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa K4683024
Lambert_GA 2023
Gel Experimentation
We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.
User Reviews
UNIQ14b23c1a5dfe0427-partinfo-00000001-QINU UNIQ14b23c1a5dfe0427-partinfo-00000002-QINU