Difference between revisions of "Part:BBa K4815016:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | ===Applications of | + | ===Applications of BBa_K4815016=== |
We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity. | We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity. | ||
<html> | <html> | ||
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===User Reviews=== | ===User Reviews=== | ||
| − | <!-- DON'T DELETE --><partinfo> | + | <!-- DON'T DELETE --><partinfo>BBa_K4815016 StartReviews</partinfo> |
<!-- Template for a user review | <!-- Template for a user review | ||
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| − | <partinfo> | + | <partinfo>BBa_K4815016 AddReview number</partinfo> |
<I>Username</I> | <I>Username</I> | ||
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<!-- End of the user review template --> | <!-- End of the user review template --> | ||
| − | <!-- DON'T DELETE --><partinfo> | + | <!-- DON'T DELETE --><partinfo>BBa_K4815016 EndReviews</partinfo> |
Latest revision as of 06:48, 12 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4815016
We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity.
User Reviews
UNIQf20bd5ef59812267-partinfo-00000001-QINU UNIQf20bd5ef59812267-partinfo-00000002-QINU