Difference between revisions of "Part:BBa K4815015:Experience"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | |||
===Applications of BBa_K4815015=== | ===Applications of BBa_K4815015=== | ||
| + | We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity. | ||
| + | <html> | ||
| + | <figure><center> | ||
| + | <img alt="" | ||
| + | src="https://static.igem.wiki/teams/4815/wiki/result/7.png" | ||
| + | width="400" | ||
| + | title=""> | ||
| + | </figure> | ||
| + | </html> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 06:35, 12 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4815015
We used a synthetic promoter to drive the expression of the YeGFP gene on the same plasmid, while the TEF1 promoter was used in the reverse orientation to drive the expression of the mCherry gene. Additionally, we incorporated a lactose-inducible switch to enhance safety. We utilized flow cytometry to monitor the two fluorescence signals excited by different light channels and analyzed the corresponding data. We plotted the natural logarithm of the ratio of GFP to mCherry (ln(GFP/mCherry)) as a frequency distribution graph to showcase the relative expression strength of different promoters in yeast. The figure bellow shows success in utilizing the plasmids in detecting fluorescence intensity.
User Reviews
UNIQ1a407f8a007981fb-partinfo-00000001-QINU UNIQ1a407f8a007981fb-partinfo-00000002-QINU