Difference between revisions of "Part:BBa K210012"
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<partinfo>BBa_K210012 short</partinfo> | <partinfo>BBa_K210012 short</partinfo> | ||
| − | This is a tandem sequence of lacI binding sites(GAAATGTGAGCGCTCACAACT)made by MPR. In the project "Time bomb" of iGEM Kyoto 2009, this part was used in order to | + | This is a tandem sequence of lacI binding sites(GAAATGTGAGCGCTCACAACT)made by MPR. In the project "Time bomb" of iGEM Kyoto 2009, this part was used in order to stabilize linear DNA. About MPR, click [http://2009.igem.org/Team:Kyoto/GSDD/Experiment here] |
It is very difficult to clone this parts because tandem sequence can induce mutation. So, if you want to use this parts, you should cloning this under 32C under which it is liower possible that mutation happens. | It is very difficult to clone this parts because tandem sequence can induce mutation. So, if you want to use this parts, you should cloning this under 32C under which it is liower possible that mutation happens. | ||
Latest revision as of 10:20, 24 October 2009
LacI binding sites' tandem sequence #1600
This is a tandem sequence of lacI binding sites(GAAATGTGAGCGCTCACAACT)made by MPR. In the project "Time bomb" of iGEM Kyoto 2009, this part was used in order to stabilize linear DNA. About MPR, click [http://2009.igem.org/Team:Kyoto/GSDD/Experiment here]
It is very difficult to clone this parts because tandem sequence can induce mutation. So, if you want to use this parts, you should cloning this under 32C under which it is liower possible that mutation happens.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]